Article
In vivo and in vitro characterization of site-specific recombination of a novel serine integrase from the temperate phage EFC-1.
Biochemical and biophysical research communications - 22 Apr 2016
Yoon Bohyun, Kim Inki, Nam Ja-Ae, Chang Hyo-Ihl, Ha Chang Hoon
Abstract excerpt
EFC-1 integrase is a site-specific recombinase that belongs to the large family of serine recombinase. In previously study, we isolated the temperate phage EFC-1, and characterized its genomic sequence. Within its genome, Orf28 was predicted encode a 464 amino acid of a putative integrase gene. In this study, EFC-1 integrase was characterized in vitro and in vivo. In vitro assay was performed using purified...
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