Article
Allosteric Activation of Escherichia coli Glucosamine-6-Phosphate Deaminase (NagB) In Vivo Justified by Intracellular Amino Sugar Metabolite Concentrations.
Journal of bacteriology - 1 Jun 2016
Álvarez-Añorve Laura I, Gaugué Isabelle, Link Hannes, Marcos-Viquez Jorge, Díaz-Jiménez Dana M, Zonszein Sergio, Bustos-Jaimes Ismael, Schmitz-Afonso Isabelle, Calcagno Mario L, Plumbridge Jacqueline
Abstract excerpt
UNLABELLED: We have investigated the impact of growth on glucosamine (GlcN) and N-acetylglucosamine (GlcNAc) on cellular metabolism by quantifying glycolytic metabolites in Escherichia coli Growth on GlcNAc increased intracellular pools of both GlcNAc6P and GlcN6P 10- to 20-fold compared to growth on glucose. Growth on GlcN produced a 100-fold increase in GlcN6P but only a slight increase in GlcNAc6P. Changes to...
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