Article
[Verification and Validation on Single Nucleotide Polymorphism Analysis of Alcohol Metabolism-Related Genes ADH1B and ALDH2, Using Dried-Saliva Samples].
Rinsho byori. The Japanese journal of clinical pathology - 1 Nov 2015
Murata Shigenori, Hayashida Mariko, Ishiguro-Tanaka Yuko, Imazeki Hiromi, Hayashi Emiko, Yokoyama Akira, Kinoshita Kenji
Abstract excerpt
We have developed a new method for unprocessed biological specimens as templates directly into the TaqMan assay. Saliva was needed to be put on a water-soluble paper and dried, because foreign substances, such as a filter paper, hinder fluorescence detection through the assay. Genotyping of alcohol metabolism-related genes ADH1B (rs1229984) and ALDH2 (rs671) polymorphisms was, subsequently, performed by TaqMan...
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