Single-tube genotyping from a human hair root by direct PCR.

doi:10.2116/analsci.25.1487

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Article

Single-tube genotyping from a human hair root by direct PCR.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry - 1 Dec 2009

Hayashida Mariko, Iwao-Koizumi Kyoko, Murata Shigenori, Kinoshita Kenji

Abstract excerpt

We have developed a simple, labor-saving, inexpensive and rapid SNP genotyping method that directly uses a human hair root as the template. This single-tube genotyping method was used to successfully and reliably genotype the ADH1B and ALDH2 polymorphisms using a hair root (without DNA isolation) and the polymerase chain reaction (PCR) enzyme kit KOD FX. Since the DNA extraction step was eliminated, the...

Topics

Adolescent
Alcohol Dehydrogenase
Aldehyde Dehydrogenase
Aldehyde Dehydrogenase, Mitochondrial
Female
Genotype
Hair
Humans
Polymerase Chain Reaction
Polymorphism, Single Nucleotide
Reproducibility of Results
Time Factors
Young Adult

Source

Open in PubMed

Identifiers

PMID: 20009340
DOI: 10.2116/analsci.25.1487

Genes

ALDH2

Provenance

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