Article
Single-tube genotyping from a human hair root by direct PCR.
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry - 1 Dec 2009
Hayashida Mariko, Iwao-Koizumi Kyoko, Murata Shigenori, Kinoshita Kenji
Abstract excerpt
We have developed a simple, labor-saving, inexpensive and rapid SNP genotyping method that directly uses a human hair root as the template. This single-tube genotyping method was used to successfully and reliably genotype the ADH1B and ALDH2 polymorphisms using a hair root (without DNA isolation) and the polymerase chain reaction (PCR) enzyme kit KOD FX. Since the DNA extraction step was eliminated, the...
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