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Article

Direct detection of single nucleotide polymorphism (SNP) by the TaqMan PCR assay using dried saliva on water-soluble paper and hair-roots, without DNA extraction.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry - 1 Jan 2014

Hayashida Mariko, Ota Tomoko, Ishii Minori, Iwao-Koizumi Kyoko, Murata Shigenori, Kinoshita Kenji

Abstract excerpt

We have developed a new method for directly using unprocessed biological specimens as templates for the TaqMan assay. DNA extraction and purification had been believed to be required for the assay, but our new method could avoid hindering fluorescence detection, even if the templates were used directly. Saliva was needed to be put on water-soluble paper and dried, and hairs were cut to be about 10 mm long. This...

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Identifiers

PMID: 24614740
DOI: 10.2116/analsci.30.427

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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