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Article

A Versatile Strategy for Isolating a Highly Enriched Population of Intestinal Stem Cells

25 Feb 2016

Abstract excerpt

The isolation of pure populations of mouse intestinal stem cells (ISCs) is essential to facilitate functional studies of tissue homeostasis, tissue regeneration, and intestinal diseases. However, the purification of ISCs has relied predominantly on the use of transgenic reporter alleles in mice. Here, we introduce a combinational cell surface marker-mediated strategy that allows the isolation of an ISC population...

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PMID: 26923822
DOI: 10.1016/j.stemcr.2016.01.014

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