Article
Hetero-oligomeric MspA pores in Mycobacterium smegmatis.
FEMS microbiology letters - 1 Apr 2016
Pavlenok Mikhail, Niederweis Michael
Abstract excerpt
The porin MspA of Mycobacterium smegmatis is a biological nanopore used for DNA sequencing. The octameric MspA pore can be isolated from M. smegmatis in milligram quantities, is extremely stable against denaturation and rapidly inserts into lipid membranes. Here, we show that MspA pores composed of different Msp subunits are formed in M. smegmatis and that hetero-oligomers of different Msp monomers increase the...
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