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Article

Easy mammalian expression and crystallography of maltose-binding protein-fused human proteins.

Journal of structural biology - 1 Apr 2016

Bokhove Marcel, Sadat Al Hosseini Hamed, Saito Takako, Dioguardi Elisa, Gegenschatz-Schmid Katharina, Nishimura Kaoru, Raj Isha, de Sanctis Daniele, Han Ling, Jovine Luca

Abstract excerpt

We present a strategy to obtain milligrams of highly post-translationally modified eukaryotic proteins, transiently expressed in mammalian cells as rigid or cleavable fusions with a mammalianized version of bacterial maltose-binding protein (mMBP). This variant was engineered to combine mutations that enhance MBP solubility and affinity purification, as well as provide crystal-packing interactions for increased...

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Identifiers

PMID: 26850170
DOI: 10.1016/j.jsb.2016.01.016
PMCID: PMC4771870

Genes

MBP

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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