Article
Nested Machine Learning Facilitates Increased Sequence Content for Large-Scale Automated High Resolution Melt Genotyping.
Scientific reports - 18 Jan 2016
Fraley Stephanie I, Athamanolap Pornpat, Masek Billie J, Hardick Justin, Carroll Karen C, Hsieh Yu-Hsiang, Rothman Richard E, Gaydos Charlotte A, Wang Tza-Huei, Yang Samuel
Abstract excerpt
High Resolution Melt (HRM) is a versatile and rapid post-PCR DNA analysis technique primarily used to differentiate sequence variants among only a few short amplicons. We recently developed a one-vs-one support vector machine algorithm (OVO SVM) that enables the use of HRM for identifying numerous short amplicon sequences automatically and reliably. Herein, we set out to maximize the discriminating power of...
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