Article
High-resolution melt PCR analysis for genotyping of Ureaplasma parvum isolates directly from clinical samples.
Journal of clinical microbiology - 1 Feb 2014
Payne Matthew S, Tabone Tania, Kemp Matthew W, Keelan Jeffrey A, Spiller O Brad, Newnham John P
Abstract excerpt
Ureaplasma sp. infection in neonates and adults underlies a variety of disease pathologies. Of the two human Ureaplasma spp., Ureaplasma parvum is clinically the most common. We have developed a high-resolution melt (HRM) PCR assay for the differentiation of the four serovars of U. parvum in a single step. Currently U. parvum strains are separated into four serovars by sequencing the promoter and coding region of...
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