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Article

A high-resolution melt curve toolkit to identify lineage-defining SARS-CoV-2 mutations.

Scientific reports - 8 Mar 2023

Fraser Alice J, Greenland-Bews Caitlin, Kelly Daniel, Williams Christopher T, Body Richard, Adams Emily R, Atienzar Ana Cubas, Edwards Thomas, Allen David J

Abstract excerpt

The emergence of severe acute respiratory syndrome 2 (SARS-CoV-2) variants of concern (VOCs), with mutations linked to increased transmissibility, vaccine escape and virulence, has necessitated the widespread genomic surveillance of SARS-CoV-2. This has placed a strain on global sequencing capacity, especially in areas lacking the resources for large scale sequencing activities. Here we have developed three...

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PMID: 36890186
DOI: 10.1038/s41598-023-30754-1
PMCID: PMC9994400

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