Article
Sensitive deep-sequencing-based HIV-1 genotyping assay to simultaneously determine susceptibility to protease, reverse transcriptase, integrase, and maturation inhibitors, as well as HIV-1 coreceptor tropism.
Antimicrobial agents and chemotherapy - 1 Jan 2014
Gibson Richard M, Meyer Ashley M, Winner Dane, Archer John, Feyertag Felix, Ruiz-Mateos Ezequiel, Leal Manuel, Robertson David L, Schmotzer Christine L, Quiñones-Mateu Miguel E
Abstract excerpt
With 29 individual antiretroviral drugs available from six classes that are approved for the treatment of HIV-1 infection, a combination of different phenotypic and genotypic tests is currently needed to monitor HIV-infected individuals. In this study, we developed a novel HIV-1 genotypic assay based on deep sequencing (DeepGen HIV) to simultaneously assess HIV-1 susceptibilities to all drugs targeting the three...
Topics
- Anti-HIV Agents
- Genotype
- HIV-1
- High-Throughput Nucleotide Sequencing
- Humans
- Integrases
- Polymerase Chain Reaction
- RNA-Directed DNA Polymerase
- Receptors, HIV