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Article

Generation of Knock-In Pigs Carrying Oct4-tdTomato Reporter through CRISPR/Cas9-Mediated Genome Engineering.

PloS one - 1 Jan 2016

Lai Sisi, Wei Shu, Zhao Bentian, Ouyang Zhen, Zhang Quanjun, Fan Nana, Liu Zhaoming, Zhao Yu, Yan Quanmei, Zhou Xiaoqing, Li Li, Xin Jige, Zeng Yangzhi, Lai Liangxue, Zou Qingjian

Abstract excerpt

The porcine pluripotent cells that can generate germline chimeras have not been developed. The Oct4 promoter-based fluorescent reporter system, which can be used to monitor pluripotency, is an important tool to generate authentic porcine pluripotent cells. In this study, we established a porcine Oct4 reporter system, wherein the endogenous Oct4 promoter directly controls red fluorescent protein (RFP). 2A-tdTomato...

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Identifiers

PMID: 26756580
DOI: 10.1371/journal.pone.0146562
PMCID: PMC4710570

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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