Article
Development of a sensitive method for directly sequencing GII.4 norovirus genome.
Diagnostic microbiology and infectious disease - 1 Mar 2016
Xue Liang, Cai Weicheng, Wu Qingping, Kou Xiaoxia, Zhang Jumei, Guo Weipeng
Abstract excerpt
A new strategy was proposed for amplifying and sequencing GII.4 norovirus genomes directly. A set of primer pairs was rationally designed, which amplified 6 overlapping fragments encompassing the whole genome. The sensitivity of new primers was comparable to that of detection primers, and 10 viral genome sequences were successfully obtained.
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