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CRISPR-Cas12a combined with reverse transcription recombinase polymerase amplification for sensitive and specific detection of human norovirus genotype GII.4.

Virology - 1 Dec 2021

Qian Weidong, Huang Jie, Wang Xuefei, Wang Ting, Li Yongdong

Abstract excerpt

Human norovirus (NOV) is a common and serious virus that accounts for sporadic cases and outbreaks of gastroenteritis. This study aimed to develop rapid, reliable and portable detection systems by coupling reverse transcription recombinase polymerase amplification (RT-RPA) with CRISPR-Cas12a (RT-RPA-Cas12a) for NOV genotype GII.4. Here, three primers for RNA-dependent RNA polymerase gene of NOV were designed and...

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PMID: 34601182
DOI: 10.1016/j.virol.2021.09.008

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