Article
A simple and novel method for GII norovirus genome clone with generic primers.
Journal of applied microbiology - 1 Aug 2013
Xue L, Wu Q, Kou X, Zhang J, Guo W
Abstract excerpt
AIMS: This study aims to establish a novel method for cloning GII norovirus genome using generic primers rationally designed based on multiple alignments of 96 GII norovirus genome sequences. METHODS AND RESULTS: Based on conservative analysis of 96 GII norovirus genome sequences available in GenBank, three fragments encompassing the full-length genome were rationally designed. Fragments A, B and C were amplified...
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