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Article

Control for stochastic sampling variation and qualitative sequencing error in next generation sequencing

29 Aug 2015

Abstract excerpt

BACKGROUND: Clinical implementation of Next-Generation Sequencing (NGS) is challenged by poor control for stochastic sampling, library preparation biases and qualitative sequencing error. To address these challenges we developed and tested two hypotheses. METHODS: Hypothesis 1: Analytical variation in quantification is predicted by stochastic sampling effects at input of a) amplifiable nucleic acid target...

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PMID: 26693143
DOI: 10.1016/j.bdq.2015.08.003

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