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Random Tagging Genotyping by Sequencing (rtGBS), an Unbiased Approach to Locate Restriction Enzyme Sites across the Target Genome.

PloS one - 1 Jan 2015

Hilario Elena, Barron Lorna, Deng Cecilia H, Datson Paul M, De Silva Nihal, Davy Marcus W, Storey Roy D

Abstract excerpt

UNLABELLED: Genotyping by sequencing (GBS) is a restriction enzyme based targeted approach developed to reduce the genome complexity and discover genetic markers when a priori sequence information is unavailable. Sufficient coverage at each locus is essential to distinguish heterozygous from homozygous sites accurately. The number of GBS samples able to be pooled in one sequencing lane is limited by the number of...

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Identifiers

PMID: 26633193
DOI: 10.1371/journal.pone.0143193
PMCID: PMC4669186

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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