Article
Structure-function relationships in Gan42B, an intracellular GH42 β-galactosidase from Geobacillus stearothermophilus.
Acta crystallographica. Section D, Biological crystallography - 1 Dec 2015
Solomon Hodaya V, Tabachnikov Orly, Lansky Shifra, Salama Rachel, Feinberg Hadar, Shoham Yuval, Shoham Gil
Abstract excerpt
Geobacillus stearothermophilus T-6 is a Gram-positive thermophilic soil bacterium that contains a battery of degrading enzymes for the utilization of plant cell-wall polysaccharides, including xylan, arabinan and galactan. A 9.4 kb gene cluster has recently been characterized in G. stearothermophilus that encodes a number of galactan-utilization elements. A key enzyme of this degradation system is Gan42B, an...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Catalytic Domain
- Crystallography, X-Ray
- Escherichia coli
- Galactose
- Gene Expression
- Geobacillus stearothermophilus
- Kinetics
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Nitrophenylgalactosides