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Article

Performance of a one-step quantitative duplex RT-PCR for detection of rotavirus A and noroviruses GII during two periods of high viral circulation.

Journal of virological methods - 1 Feb 2016

Fumian Tulio M, Leite José Paulo G, Rocha Mônica S, de Andrade Juliana S R, Fioretti Julia M, de Assis Rosane M S, Assis Matheus R S, Fialho Alexandre M, Miagostovich Marize P

Abstract excerpt

Rotavirus A (RVA) and noroviruses (NoV) are the major viral agents of acute gastroenteritis (AGE) worldwide. In the present study, we aimed to evaluate the performance of a one-step duplex quantitative RT-PCR (dRT-qPCR) assay, established for detection and quantification of RVA and NoV genogroup II (GII) using a single DNA standard curve (SC), as well as to investigate the association between fecal viral load and...

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PMID: 26611226
DOI: 10.1016/j.jviromet.2015.11.008

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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