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Article

Single-step RT-PCR assay for dual genotyping of GI and GII norovirus strains.

Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology - 1 Jan 2021

Chhabra Preeti, Browne Hannah, Huynh Thalia, Diez-Valcarce Marta, Barclay Leslie, Kosek Margaret N, Ahmed Tahmeed, Lopez Maria Renee, Pan Chao-Yang, Vinjé Jan

Abstract excerpt

BACKGROUND: Noroviruses are the major cause of acute gastroenteritis (AGE) in people of all ages globally. Standardized genotyping is key for outbreak investigations and surveillance networks. OBJECTIVE: Here we describe the validation of a one-step conventional RT-PCR assay for sequence-based dual typing of GI and GII noroviruses. This polymerase (P) and capsid (C) dual typing assay uses a combination of...

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Identifiers

PMID: 33260046
DOI: 10.1016/j.jcv.2020.104689
PMCID: PMC7816162

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Source receipt: pubmed, retrieved 7 Sept 2026.

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