Article
Altered Escherichia coli membrane protein assembly machinery allows proper membrane assembly of eukaryotic protein vitamin K epoxide reductase.
Proceedings of the National Academy of Sciences of the United States of America - 8 Dec 2015
Hatahet Feras, Blazyk Jessica L, Martineau Eugenie, Mandela Eric, Zhao Yongxin, Campbell Robert E, Beckwith Jonathan, Boyd Dana
Abstract excerpt
Functional overexpression of polytopic membrane proteins, particularly when in a foreign host, is often a challenging task. Factors that negatively affect such processes are poorly understood. Using the mammalian membrane protein vitamin K epoxide reductase (VKORc1) as a reporter, we describe a genetic selection approach allowing the isolation of Escherichia coli mutants capable of functionally expressing this...
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