Article
Generation of Loss-of-Function Mutants for Wheat Rust Disease Resistance Gene Cloning.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017
Mago Rohit, Till Bradley, Periyannan Sambasivam, Yu Guotai, Wulff Brande B H, Lagudah Evans
Abstract excerpt
One of the most important tools to identify and validate rust resistance gene function is by producing loss-of-function mutants. Mutants can be produced using irradiation, chemicals, and insertions. Among all the mutagens, ethyl methanesulfonate (EMS) and sodium azide are most favored because of the ease of use and generation of random point mutations in the genome. The mutants so produced facilitate the...
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