Article
Characterization of the Interaction between the Matrix Protein of Vesicular Stomatitis Virus and the Immunoproteasome Subunit LMP2.
Journal of virology - 1 Nov 2015
Beilstein Frauke, Obiang Linda, Raux Hélène, Gaudin Yves
Abstract excerpt
UNLABELLED: The matrix protein (M) of vesicular stomatitis virus (VSV) is involved in virus assembly, budding, gene regulation, and cellular pathogenesis. Using a yeast two-hybrid system, the M globular domain was shown to interact with LMP2, a catalytic subunit of the immunoproteasome (which replaces the standard proteasome catalytic subunit PSMB6). The interaction was validated by coimmunoprecipitation of M and...
Topics
- Base Sequence
- Blotting, Western
- Cysteine Endopeptidases
- HeLa Cells
- Humans
- Immunoprecipitation
- Molecular Sequence Data
- Mutation
- Protein Binding
- Sequence Alignment
- Sequence Analysis, DNA
- Two-Hybrid System Techniques
- Vesiculovirus
- Viral Matrix Proteins