Article
High-resolution melting analysis as an alternative method for human neutrophil antigen genotyping.
Immunohematology - 1 Jan 2015
Yasui Kazuta, Tanaka Mitsunobu, Hayashi Tomoya, Matsuyama Nobuki, Kuroishi Ayumu, Furuta Rika A, Tani Yoshihiko, Hirayama Fumiya
Abstract excerpt
Human neutrophil antigen (HNA)-typed granulocyte panels are widely used to screen for the presence of HNA antibodies and to determine antibody specificity. Many laboratories screen donors for HNA genotypes using low-throughput methods such as allele-specific polymerase chain reaction (PCR), PCR-restriction fragment-length polymorphism, and multiplex PCR. In the present study, we used a high-resolution melting...
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