Article
Allelic Discrimination by TaqMan-PCR for Genotyping of Human Neutrophil Antigens.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015
Steffensen Rudi, Baech John, Nielsen Kaspar R
Abstract excerpt
Neutrophil antigens are implicated in a variety of clinical conditions, including neonatal immune neutropenia, transfusion-related acute lung injury, refractoriness to granulocyte transfusions, febrile transfusion reactions, and autoimmune neutropenia. In this report, we describe simultaneous genotyping of human neutrophil antigens (HNA)-1, -3, -4, and -5 using PCR with allele-specific TaqMan probes and end-point...
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