Article
Cellulose hydrolysis ability of a Clostridium thermocellum cellulosome containing small-size scaffolding protein CipA.
Journal of biotechnology - 20 Oct 2015
Deng Lan, Mori Yutaka, Sermsathanaswadi Junjarus, Apiwatanapiwat Waraporn, Kosugi Akihiko
Abstract excerpt
Mutant Clostridium thermocellum YM72 that produces small-size scaffolding protein CipA (ssCipA) was isolated from wild-type YM4. Sequencing of ssCipA revealed that two domains, cohesin 6 and cohesin 7, were not present. Cellulosome prepared from YM72 exhibited a significant reduction of hydrolysis ability on crystalline celluloses such as Sigmacell type-20 and cellulose from Halocynthia. To investigate this...
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