Article
Whole-animal mounts of Caenorhabditis elegans for 3D imaging using atomic force microscopy.
Nanomedicine : nanotechnology, biology, and medicine - 1 Nov 2015
Allen Michael J, Kanteti Rajani, Riehm Jacob J, El-Hashani Essam, Salgia Ravi
Abstract excerpt
The 3D surface of Caenorhabditis elegans was imaged at nanometer resolution using atomic force microscopy (AFM). Oscillation of a medium stiffness silicon AFM cantilever at the upper second amplitude peak, typically 6 times above the fundamental frequency, vastly improved image quality on the moist, sticky, and soft worms. Whole-animal mounts of normal and double-headed mutants of the nematode worm were prepared...
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