Article
A Quantitative System for Studying Metastasis Using Transparent Zebrafish.
Cancer research - 15 Oct 2015
Heilmann Silja, Ratnakumar Kajan, Langdon Erin, Kansler Emily, Kim Isabella, Campbell Nathaniel R, Perry Elizabeth, McMahon Amy, Kaufman Charles, van Rooijen Ellen, Lee William, Iacobuzio-Donahue Christine, Hynes Richard, Zon Leonard, Xavier Joao, White Richard
Abstract excerpt
Metastasis is the defining feature of advanced malignancy, yet remains challenging to study in laboratory environments. Here, we describe a high-throughput zebrafish system for comprehensive, in vivo assessment of metastatic biology. First, we generated several stable cell lines from melanomas of transgenic mitfa-BRAF(V600E);p53(-/-) fish. We then transplanted the melanoma cells into the transparent casper strain...
Topics
- Algorithms
- Animals
- Animals, Genetically Modified
- Cell Line, Tumor
- Disease Models, Animal
- Gene Expression Profiling
- Melanoma
- Models, Biological
- Mutation
- Neoplasm Metastasis
- Neoplasms
- Transcriptome