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Colorimetric detection of both total genomic and loci-specific DNA methylation from limited DNA inputs

10 Jul 2015

Abstract excerpt

BACKGROUND: Aberrant DNA methylation marks are potential disease biomarkers, and detecting both total genomic and gene-specific DNA methylation can aid in clinical decisions. While a plethora of methods exist in research, simpler, more convenient alternatives are needed to enhance both routine diagnostics and research. RESULTS: Herein, we describe colorimetric assays using methyl-binding domain (MBD) proteins for...

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PMID: 26167236
DOI: 10.1186/s13148-015-0100-6

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