Article
PCR analysis of half-seeds of cereal crops and its application in marker-assisted selection and breeding.
Chinese journal of biotechnology - 1 Jan 1996
Zhai W, Lu C, Zhu L, Yang W, Zhang Q
Abstract excerpt
A simple and rapid PCR method was adapted to DNA analysis of cereal seeds. Half-seeds of rice, wheat, and maize were treated with an extraction buffer and the resulting supernatants were used in PCR and RAPD reactions. PCR products amplified from the half-seed DNA extracts were identical with that from leaf tissue DNA extracts when the same specific primers were used in PCR reactions. The remaining half-seeds...
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