Article
Characterization of the First Fungal Glycosyl Hydrolase Family 19 Chitinase (NbchiA) from Nosema bombycis (Nb).
The Journal of eukaryotic microbiology - 1 Jan 2000
Han Bing, Zhou Kang, Li Zhihong, Sun Bin, Ni Qi, Meng Xianzhi, Pan Guoqing, Li Chunfeng, Long Mengxian, Li Tian, Zhou Congzhao, Li Weifang, Zhou Zeyang
Abstract excerpt
Chitinases (EC 3.2.1.14), as one kind of glycosyl hydrolase, hydrolyze the β-(1,4) linkages of chitin. According to the sequence similarity, chitinases can be divided into glycoside hydrolase family 18 and family 19. Here, a chitinase from Nosema bombycis (NbchiA) was cloned and purified by metal affinity chromatography and molecular exclusion chromatography. Sequence analysis indicated that NbchiA belongs to...
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