Article
Crystal structure and enzymatic properties of a bacterial family 19 chitinase reveal differences from plant enzymes.
The FEBS journal - 1 Nov 2006
Hoell Ingunn A, Dalhus Bjørn, Heggset Ellinor B, Aspmo Stein I, Eijsink Vincent G H
Abstract excerpt
We describe the cloning, overexpression, purification, characterization and crystal structure of chitinase G, a single-domain family 19 chitinase from the Gram-positive bacterium Streptomyces coelicolor A3(2). Although chitinase G was not capable of releasing 4-methylumbelliferyl from artificial chitooligosaccharide substrates, it was capable of degrading longer chitooligosaccharides at rates similar to those...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Catalytic Domain
- Chitin
- Chitinases
- Crystallization
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Oligosaccharides
- Plant Proteins