Article
Determination of agmatine using isotope dilution UPLC-tandem mass spectrometry: application to the characterization of the arginine decarboxylase pathway in Pseudomonas aeruginosa.
Analytical and bioanalytical chemistry - 1 Jul 2015
Dalluge Joseph J, McCurtain Jennifer L, Gilbertsen Adam J, Kalstabakken Kyle A, Williams Bryan J
Abstract excerpt
A method has been developed for the direct determination of agmatine in bacterial culture supernatants using isotope dilution ultra performance liquid chromatography (UPLC)-tandem mass spectrometry (UPLC-MS/MS). Agmatine determination in bacterial supernatants is comprised of spiking culture or isolate supernatants with a fixed concentration of uniformly labeled (13)C5,(15)N4-agmatine (synthesized by...
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