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Hygromycin B-induced cell death is partly mediated by reactive oxygen species in rice (Oryza sativa L.).

Plant molecular biology - 1 Dec 2015

Oung Hui-Min, Lin Ke-Chun, Wu Tsung-Meng, Chandrika Nulu Naga Prafulla, Hong Chwan-Yang

Abstract excerpt

The aminoglycoside antibiotic hygromycin B (Hyg) inhibits prokaryotic, chloroplast and mitochondrial protein synthesis. Because of the toxic effect of Hyg on plant cells, the HPT gene, encoding hygromycin phosphotransferase, has become one of the most widely used selectable markers in plant transformation. Yet the mechanism behind Hyg-induced cell lethality in plants is not clearly understood. In this study, we...

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PMID: 26415870
DOI: 10.1007/s11103-015-0380-4

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