Article
Duffy blood group phenotype-genotype correlations using high-resolution melting analysis PCR and microarray reveal complex cases including a new null FY*A allele: the role for sequencing in genotyping algorithms.
Vox sanguinis - 1 Oct 2015
Lopez G H, Morrison J, Condon J A, Wilson B, Martin J R, Liew Y-W, Flower R L, Hyland C A
Abstract excerpt
BACKGROUND AND OBJECTIVES: Duffy blood group phenotypes can be predicted by genotyping for single nucleotide polymorphisms (SNPs) responsible for the Fy(a) /Fy(b) polymorphism, for weak Fy(b) antigen, and for the red cell null Fy(a-b-) phenotype. This study correlates Duffy phenotype predictions with serotyping to assess the most reliable procedure for typing. MATERIALS AND METHODS: Samples, n = 155 (135 donors...
Topics
- Algorithms
- Alleles
- Base Sequence
- Duffy Blood-Group System
- Genetic Association Studies
- Humans
- Molecular Sequence Data
- Oligonucleotide Array Sequence Analysis
- Phase Transition
- Polymerase Chain Reaction
- Polymorphism, Single Nucleotide
- Receptors, Cell Surface