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Measuring the elasticity of ribonucleotide(s)-containing DNA molecules using AFM.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015

Koh Kyung Duk, Chiu Hsiang-Chih, Riedo Elisa, Storici Francesca

Abstract excerpt

Ribonucleotides, ribonucleoside monophosphates (rNMPs), have been revealed as possibly the most noncanonical nucleotides in genomic DNA. rNMPs, either not removed from Okazaki fragments during DNA replication or incorporated and scattered throughout the genome, pose a perturbation to the structure and a threat to the stability of DNA. The instability of DNA is mainly due to the extra 2'-hydroxyl (OH) group of...

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PMID: 25895994
DOI: 10.1007/978-1-4939-2562-9_3

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AFM

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