Article
Estimating Protistan Diversity Using High-Throughput Sequencing.
The Journal of eukaryotic microbiology - 1 Jan 2000
Hu Sarah K, Liu Zhenfeng, Lie Alle A Y, Countway Peter D, Kim Diane Y, Jones Adriane C, Gast Rebecca J, Cary S Craig, Sherr Evelyn B, Sherr Barry F, Caron David A
Abstract excerpt
Sequencing hypervariable regions from the 18S rRNA gene is commonly employed to characterize protistan biodiversity, yet there are concerns that short reads do not provide the same taxonomic resolution as full-length sequences. A total of 7,432 full-length sequences were used to perform an in silico analysis of how sequences of various lengths and target regions impact downstream ecological interpretations....
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