Article
Environmental rRNA inventories miss over half of protistan diversity.
BMC microbiology - 16 Dec 2008
Jeon Sunok, Bunge John, Leslin Chesley, Stoeck Thorsten, Hong Sunhee, Epstein Slava S
Abstract excerpt
BACKGROUND: The main tool to discover novel microbial eukaryotes is the rRNA approach. This approach has important biases, including PCR discrimination against certain rRNA gene species, which makes molecular inventories skewed relative to the source communities. The degree of this bias has not been quantified, and it remains unclear whether species missed from clone libraries could be recovered by increasing...
Topics
- Animals
- DNA Primers
- DNA, Protozoan
- DNA, Ribosomal
- Eukaryota
- Genetic Variation
- Phylogeny
- RNA, Ribosomal
- Sequence Analysis, DNA