Article
Placing environmental next-generation sequencing amplicons from microbial eukaryotes into a phylogenetic context.
Molecular biology and evolution - 1 Apr 2014
Dunthorn Micah, Otto Johannes, Berger Simon A, Stamatakis Alexandros, Mahé Frédéric, Romac Sarah, de Vargas Colomban, Audic Stéphane, Stock Alexandra, Kauff Frank, Stoeck Thorsten
Abstract excerpt
Nucleotide positions in the hypervariable V4 and V9 regions of the small subunit (SSU)-rDNA locus are normally difficult to align and are usually removed before standard phylogenetic analyses. Yet, with next-generation sequencing data, amplicons of these regions are all that are available to answer ecological and evolutionary questions that rely on phylogenetic inferences. With ciliates, we asked how inclusion of...
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