Article
Optimization of the Divergent method for genotyping single nucleotide variations using SYBR Green-based single-tube real-time PCR.
Mutation research - 1 Jan 2000
Gentilini Fabio, Turba Maria E
Abstract excerpt
A novel technique, called Divergent, for single-tube real-time PCR genotyping of point mutations without the use of fluorescently labeled probes has recently been reported. This novel PCR technique utilizes a set of four primers and a particular denaturation temperature for simultaneously amplifying two different amplicons which extend in opposite directions from the point mutation. The two amplicons can readily...
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