Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Optimization of the Divergent method for genotyping single nucleotide variations using SYBR Green-based single-tube real-time PCR.

Mutation research - 1 Jan 2000

Gentilini Fabio, Turba Maria E

Abstract excerpt

A novel technique, called Divergent, for single-tube real-time PCR genotyping of point mutations without the use of fluorescently labeled probes has recently been reported. This novel PCR technique utilizes a set of four primers and a particular denaturation temperature for simultaneously amplify...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Benzothiazoles
Share to Calibration
Share to Diamines

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Dog Diseases

Share to Fluorescent Dyes

Share to Nucleic Acid Denaturation

Share to Organic Chemicals

Share to Quinolines

Source

Identifiers

PMID: 25847266
DOI: 10.1016/j.mrfmmm.2014.05.013

Genes

DNM1

Variants

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-25847266-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Optimization of the Divergent method for genotyping single nucleotide variations using SYBR Green-based single-tube real-time PCR.PMID 25847266Read ↗

Two novel real-time PCR methods for genotyping the von Willebrand disease type I mutation in Doberman Pinscher dogs.Semantic proximity Read ↗

Defining the performance parameters of a rapid screening tool for myotonic dystrophy type 1 based on triplet-primed PCR and melt curve analysis.Semantic proximity Read ↗

A novel simple genotyping assay for detection of the 'Gait keeper' mutation in DMRT3 and allele frequencies in Azteca and Costa Rican Saddle Horse breeds.Semantic proximity Read ↗

Analysis of multiple single nucleotide polymorphisms closely positioned in the ovine PRNP gene using linear fluorescent probes and melting curve analysis.Semantic proximity Read ↗

Temperature switch PCR (TSP): Robust assay design for reliable amplification and genotyping of SNPs.Semantic proximity Read ↗

Presence and impact of the exercise-induced collapse associated DNM1 mutation in Labrador retrievers and other breeds.Semantic proximity Read ↗

Genotyping single point mutations in rd1 and rd8 mice using melting curve analysis of qPCR fragments.Semantic proximity Read ↗

Detection of KIT Genotype in Pigs by TaqMan MGB Real-Time Quantitative Polymerase Chain Reaction.Semantic proximity Read ↗

Incidence of the mask phenotype M264V mutation in Labrador Retrievers.Semantic proximity Read ↗

Short Communication: Ligase Detection Reaction as a genotyping technique to identify cats carrying the c.1024G>T mutation on TRPV4 geneSemantic proximity Read ↗

A novel rapid genotyping technique for Collie eye anomaly: SYBR Green-based real-time polymerase chain reaction method applicable to blood and saliva specimens on Flinders Technology Associates filter paper.Semantic proximity Read ↗

Temperature switch PCR (TSP): a gel-based molecular marker technique for investigating single nucleotide polymorphisms.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.