Article
Temperature switch PCR (TSP): a gel-based molecular marker technique for investigating single nucleotide polymorphisms.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2014
Thanh Le Phuoc, Khoo Kelvin
Abstract excerpt
Temperature Switch PCR (TSP) is a robust single-marker single nucleotide polymorphism (SNP) genotyping technique with broad applications in genetic studies of various organisms. The technique consists of a biphasic PCR with two sets of primers, a locus-specific set and a nested locus-specific set. The PCR products can be easily assessed for polymorphism based on different band sizes using agarose gel electrophoresis.
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