Article
A novel simple genotyping assay for detection of the 'Gait keeper' mutation in DMRT3 and allele frequencies in Azteca and Costa Rican Saddle Horse breeds.
Molecular and cellular probes - 1 Apr 2020
Ayala-Valdovinos Miguel Angel, Galindo-García Jorge, Sánchez-Chiprés David, Duifhuis-Rivera Theodor, Anguiano-Estrella Rubén
Abstract excerpt
The 'Gait keeper' mutation in the DMRT3 gene alters locomotion and gait patterns in horses. This mutation (C>A) has been found in all gaited breeds of horses analyzed but is absent in most non-gaited breeds. We developed a new mutagenically separated polymerase chain reaction (MS-PCR) based method for simple detection of horse DMRT3 genotype. Our method was applied in a preliminary study to determine DMRT3 allele...
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