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Article

Validation of a fast and low-cost alkaline lysis method for gDNA extraction in a pharmacogenetic context.

Cancer chemotherapy and pharmacology - 1 May 2015

Ramos-Díaz Ruth, Gutiérrez-Nicolás Fernando, Nazco-Casariego Gloria J, González-Perera Itamar, Pérez-Pérez José A

Abstract excerpt

PURPOSE: Translation of pharmacogenetic findings from the research laboratory to the clinical practice demands simple and efficient procedures. In this sense, we evaluated the suitability of a modified protocol for genomic DNA extraction based on alkaline lysis of cells. METHODS: Dried blood samples were obtained from 48 patients diagnosed with colorectal cancer. A total of 11 mutations in the dihydropyrimidine...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 25804843
DOI: 10.1007/s00280-015-2729-4

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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