Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

A method to discriminate between closely related bovine major histocompatibility complex class I alleles by combining established PCR-SSP assays with RFLPs.

Tissue antigens - 1 Apr 2015

Svitek N, Nzau B, Steinaa L, Nene V

Abstract excerpt

We have developed a polymerase chain reaction-sequence-specific primers-restriction fragment length polymorphism (PCR-SSP-RFLP) method to rapidly differentiate between the A18 and A18 variant (v) BoLA haplotypes and between A14 and A15/A15v BoLA haplotypes in Holstein/Friesian cattle. We used published SSP to PCR amplify BoLA alleles expressed in animals of known haplotype and exposed the amplicons to the...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cattle
Share to Deoxyribonucleases, Type II Site-Specific

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to DNA Primers

Share to Histocompatibility Antigens Class I

Share to Polymorphism, Restriction Fragment Length

Share to Sequence Alignment

Share to Sequence Homology, Amino Acid

Source

Identifiers

PMID: 25789713
DOI: 10.1111/tan.12524

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

A method to discriminate between closely related bovine major histocompatibility complex class I alleles by combining established PCR-SSP assays with RFLPs.PMID 25789713Read ↗

DNA typing for BoLA class I using sequence-specific primers (PCR-SSP).Semantic proximity Read ↗

Establishment of a sequence-based typing system for BoLA-DQA1 exon 2.Semantic proximity Read ↗

Extensive polymorphism of the BoLA-DRB3 gene distinguished by PCR-RFLP.Semantic proximity Read ↗

Genotyping BoLA-DRB3 alleles in Brazilian Dairy Gir cattle (Bos indicus) by temperature-gradient gel electrophoresis (TGGE) and direct sequencing.Semantic proximity Read ↗

Denaturing gradient gel electrophoresis: a rapid method for differentiating BoLA-DRB3 alleles.Semantic proximity Read ↗

First characterization of major histocompatibility complex class II DRB3 diversity in cattle breeds raised in Egypt.Semantic proximity Read ↗

Characterization of 18 new BoLA-DRB3 alleles.Semantic proximity Read ↗

High-throughput detection of bovine leukocyte antigen (BoLA)-DRB3 alleles associated with resistance to bovine leukemia virus using a rapid allele-specific PCR assay.Semantic proximity Read ↗

PCR based RFLP genotyping of bovine lymphocyte antigen DRB3.2 in Iranian Holstein population.Semantic proximity Read ↗

Characterization of BoLA class II DQA and DQB by PCR-RFLP, cloning, and sequencing reveals sequence diversity in crossbred cattle.Semantic proximity Read ↗

Short communication: characterization of DRB3 alleles in the MHC of Japanese shorthorn cattle by polymerase chain reaction-sequence-based typing.Semantic proximity Read ↗

Identification and diversity of bovine major histocompatibility complex class II haplotypes in Japanese Black and Holstein cattle in Japan.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.