Article
PCR based RFLP genotyping of bovine lymphocyte antigen DRB3.2 in Iranian Holstein population.
Pakistan journal of biological sciences : PJBS - 1 Feb 2007
Pashmi Morteza, Qanbari Saber, Ghorashi Seyed Ali, Salehi Abdolreza
Abstract excerpt
Major Histocompatibility Complex (MHC) class II locus DRB3 was investigated by PCR based restriction fragment length polymorphism (PCR-RFLP) assay. A total of 262 Holstein cows participating in the national recording system were sampled from 10 herds. A two-step polymerase chain reaction was carried out in order to amplify a 284 base-pair fragment of exon 2 of the target gene. Second PCR products were treated...
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