Article
Establishment of a sequence-based typing system for BoLA-DQA1 exon 2.
Tissue antigens - 1 Feb 2007
Takeshima S, Miki A, Kado M, Aida Y
Abstract excerpt
In cattle, bovine leukocyte antigens (BoLAs) have been extensively used as markers for bovine diseases and immunological traits. Here, we developed a rapid, high-resolution sequence-based typing (SBT) system for BoLA-DQA1. We amplified 355 bp of BoLA-DQA1 by fully nested polymerase chain reaction (PCR) using the newly constructed primers and then performed direct sequencing of each product. Using this method, we...
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