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Article

Improved thermostability of bacillus circulans cyclodextrin glycosyltransferase by the introduction of a salt bridge.

Proteins - 1 Jan 2004

Leemhuis Hans, Rozeboom Henriëtte J, Dijkstra Bauke W, Dijkhuizen Lubbert

Abstract excerpt

Cyclodextrin glycosyltransferase (CGTase) catalyzes the formation of cyclodextrins from starch. Among the CGTases with known three-dimensional structure, Thermoanaerobacterium thermosulfurigenes CGTase has the highest thermostability. By replacing amino acid residues in the B-domain of Bacillus circulans CGTase with those from T. thermosulfurigenes CGTase, we identified a B. circulans CGTase mutant (with N188D...

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PMID: 14705029
DOI: 10.1002/prot.10516

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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