Article
Site-directed mutations in tyrosine 195 of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 affect activity and product specificity.
Biochemistry - 14 Mar 1995
Penninga D, Strokopytov B, Rozeboom H J, Lawson C L, Dijkstra B W, Bergsma J, Dijkhuizen L
Abstract excerpt
Tyrosine 195 is located in the center of the active site cleft of cyclodextrin glycosyltransferase (EC 2.4.1.19) from Bacillus circulans strain 251. Alignment of amino acid sequences of CGTases and alpha-amylases, and the analysis of the binding mode of the substrate analogue acarbose in the active site cleft [Strokopytov, B., et al. (1995) Biochemistry 34, (in press)], suggested that Tyr195 plays an important...
Topics
- Amino Acid Sequence
- Bacillus
- Base Sequence
- Binding Sites
- Crystallography, X-Ray
- DNA, Bacterial
- Escherichia coli
- Glucosyltransferases
- Models, Molecular
- Molecular Sequence Data