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Article

Technical reproducibility of single-nucleotide and size-based DNA biomarker assessment using DNA extracted from formalin-fixed, paraffin-embedded tissues.

The Journal of molecular diagnostics : JMD - 1 May 2015

Zhang Shenli, Tan Iain B, Sapari Nur S, Grabsch Heike I, Okines Alicia, Smyth Elizabeth C, Aoyama Toru, Hewitt Lindsay C, Inam Imran, Bottomley Dan, Nankivell Matthew, Stenning Sally P, Cunningham David, Wotherspoon Andrew, Tsuburaya Akira, Yoshikawa Takaki, Soong Richie, Tan Patrick

Abstract excerpt

DNA extracted from formalin-fixed, paraffin-embedded (FFPE) tissues has been used in the past to analyze genetic polymorphisms. We evaluated the technical reproducibility of different types of assays for gene polymorphisms using DNA extracted from FFPE material. By using the MassARRAY iPLEX system, we investigated polymorphisms in DPYD (rs1801159 and rs3918290), UMPS (rs1801019), ERCC1 (rs11615), ERCC1...

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Share to Formaldehyde
Share to Gene Dosage
Share to Genetic Markers
Share to Genotyping Techniques
Share to Oligonucleotide Array Sequence Analysis
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Share to Reproducibility of Results
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Source

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Identifiers

PMID: 25746798
DOI: 10.1016/j.jmoldx.2014.12.001
PMCID: PMC4411247

Genes

GSTT1
ERCC1
TYMS
DPYD
ERCC2
UMPS

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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